Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.248380 |
Chromosome: | chromosome_10 |
Location: | 2720121 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g438250 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCAGACCGAAGCCAGCCAACCGAGGTAGCA |
Internal bar code: | GCCAAGCTCCTTCGTCTGGAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 457 |
LEAP-Seq percent confirming: | 96.6811 |
LEAP-Seq n confirming: | 670 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCCGTGGAAGTAAAGGAAC |
Suggested primer 2: | GACCACCTGATATGACCGCT |