| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248398 |
| Chromosome: | chromosome 17 |
| Location: | 6657513 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g743897 | PRX7 | (1 of 3) K03386 - peroxiredoxin (alkyl hydroperoxide reductase subunit C) [EC:1.11.1.15] (E1.11.1.15, PRDX, ahpC); 2-cys Peroxiredoxin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAACATCAACCCTCCCCTTTTGACATCA |
| Internal bar code: | GGGGGGGTTCCAGCTAGGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 99.9398 |
| LEAP-Seq n confirming: | 1660 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAACCCCTCCTCCTTCCT |
| Suggested primer 2: | ACAGCGACGAGGAGGAACTA |