| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248409 |
| Chromosome: | chromosome 2 |
| Location: | 784556 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078831 | (1 of 1) IPR002059//IPR012340 - Cold-shock protein, DNA-binding // Nucleic acid-binding, OB-fold | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCACGAATCCGCGGCTGCTGTCTCGCT |
| Internal bar code: | CATCGTTTAGTTTGCTTCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.7032 |
| LEAP-Seq n confirming: | 2687 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCACCAGCACCAGCAGTA |
| Suggested primer 2: | CGCTATACTGGCAATGAGCA |