| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248433 |
| Chromosome: | chromosome 2 |
| Location: | 6326507 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115050 | RSEP3,RSE3 | (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGCAGTCCTGCCGGGGCGCGCATGTGT |
| Internal bar code: | TCGAGGTCAACCTTTTCCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 99.6804 |
| LEAP-Seq n confirming: | 3743 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCCGGCCTTTACCTTAT |
| Suggested primer 2: | GTGTGGGTTCCTCGTGACTT |