Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.248433 |
Chromosome: | chromosome_2 |
Location: | 6326508 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g115050 | RSE3,RSEP3 | Intramembrane metalloprotease | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCACCATCTGTGTGGCTGTGCCTCTGGGCC |
Internal bar code: | CGATAGATCCATCGGGAACAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 450 |
LEAP-Seq percent confirming: | 99.4326 |
LEAP-Seq n confirming: | 1402 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTGCCGGCCTTTACCTTAT |
Suggested primer 2: | GTGTGGGTTCCTCGTGACTT |