| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248457 |
| Chromosome: | chromosome 3 |
| Location: | 2940228 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163200 | (1 of 1) K15683 - NF-X1-type zinc finger protein NFXL1 (NFXL1, OZFP) | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACGATGCGAGGCGATGTGAGGCGTCCG |
| Internal bar code: | AAACAAGGGGCATCTCAGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 93.1727 |
| LEAP-Seq n confirming: | 232 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTCTGTCAGAGGCGAGC |
| Suggested primer 2: | GTGTCGTTCCATCACACCTG |