Insertion junction: LMJ.RY0402.248459_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.248459
Chromosome:chromosome 2
Location:6035370
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g112550 (1 of 1) PTHR19241//PTHR19241:SF244 - ATP-BINDING CASSETTE TRANSPORTER // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGAGCACCGATGGTGTTTATCCTATTCT
Internal bar code:GCCGATTTACAGTCTCTGCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:99.2608
LEAP-Seq n confirming:1880
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTGGTCATACCAGGCTT
Suggested primer 2:GTTGTTGACTCGGTCCAGGT