Insertion junction: LMJ.RY0402.248463_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.248463
Chromosome:chromosome 1
Location:248847
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g001650 Predicted ankryin-repeat containing protein; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGACGAGGATGATGATATGTGATGGTGC
Internal bar code:ACGGCTTGCGGTGTGCGGAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:244
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAACTCCTCCTCCTCCTT
Suggested primer 2:CTCTGCCTAGTGCAACCCTC