| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248518 |
| Chromosome: | chromosome 6 |
| Location: | 2235778 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266383 | HEL26 | (1 of 1) K18995 - ATP-dependent RNA helicase DHX29 [EC:3.6.4.13] (DHX29); DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACTGTGTGTATGTGTGTGTGTGTGTGTG |
| Internal bar code: | CTGCATTAGGATGTTGCCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 98.8235 |
| LEAP-Seq n confirming: | 504 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAACCCTGTGTTTGGCTCG |
| Suggested primer 2: | GTCATCCTCTCCCTCCTTCC |