Insertion junction: LMJ.RY0402.248518_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.248518
Chromosome:chromosome 6
Location:2235787
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g266383 HEL26 (1 of 1) K18995 - ATP-dependent RNA helicase DHX29 [EC:3.6.4.13] (DHX29); DEAD/DEAH box helicase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTTCCTTTTGCTCTCACACACACACACA
Internal bar code:GCGATAATACGGATTGGGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:88
LEAP-Seq percent confirming:5.78035
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:163
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATAACCCTGTGTTTGGCTCG
Suggested primer 2:GTCATCCTCTCCCTCCTTCC