| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248526 |
| Chromosome: | chromosome 10 |
| Location: | 2865842 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439800 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCTGTGCTGCCACGACCCCAGTCCTG |
| Internal bar code: | CTGGACAGTTTCGCACGAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.9155 |
| LEAP-Seq n confirming: | 4728 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATCAGCTCTTTGGTTGG |
| Suggested primer 2: | CATAAAAACCTCCGAAGCCA |