| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248534 |
| Chromosome: | chromosome_2 |
| Location: | 2420838 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g091500 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCAAACGCCCACGCTGCGTGTGCCCGCA |
| Internal bar code: | TGGCTCGCGGCATTCCCCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 63.8298 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGTGCATTGCTGCTTTCG |
| Suggested primer 2: | AGCATGGAGTACGAGCAGGT |