Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.248573 |
Chromosome: | chromosome_12 |
Location: | 2526835 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g506000 | DIC2,ODA6 | Flagellar outer dynein arm intermediate chain 2 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGACTGTTAGGCATGGCGTGTCTAGACCA |
Internal bar code: | TCTGCCCCTACGGGCGGGGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 643 |
LEAP-Seq percent confirming: | 98.892 |
LEAP-Seq n confirming: | 714 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAAGTACGTGCTCAGTGGC |
Suggested primer 2: | ATGGGACAGTGGTAAGGCAG |