| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248581 |
| Chromosome: | chromosome 4 |
| Location: | 2382770 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221050 | (1 of 3) PTHR12136//PTHR12136:SF47 - STEROIDOGENIC ACUTE REGULATORY PROTEIN STAR // STEROIDOGENIC ACUTE REGULATORY-LIKE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGACCACGACTGGGAGCCCTTTGACTTC |
| Internal bar code: | GGGAGTAGCCAACTGGCTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 99.4652 |
| LEAP-Seq n confirming: | 186 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTGTATTTGGGAGCTTA |
| Suggested primer 2: | CAAACGTATCCGTGTGAACG |