| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248643 |
| Chromosome: | chromosome 9 |
| Location: | 7368373 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413123 | 3'UTR | ||
| Cre09.g413132 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTAGGCGCGCAGGCGCATAGCAGCATCA |
| Internal bar code: | CATAGTACTCGAGTTATGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.6288 |
| LEAP-Seq n confirming: | 8858 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTTCCCGTCTGGTGTAT |
| Suggested primer 2: | GTGGTACGGAGGTGCATTCT |