| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248662 |
| Chromosome: | chromosome 2 |
| Location: | 1655850 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATACAGTTCGCTCGTGAAGGGTTATGAT |
| Internal bar code: | TTTCTATGCTGACAGAATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 98.9592 |
| LEAP-Seq n confirming: | 5039 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACCTGATGTATTGCACG |
| Suggested primer 2: | GCTTGTGACTACGGGTGGAT |