| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248734 |
| Chromosome: | chromosome 7 |
| Location: | 2544794 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329850 | SNRK2P,KCN5 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; snRK1 family in Chlamydomonas, subgroup 2 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACGTTGTCTGTGGGCAAACACTGTGCAC |
| Internal bar code: | GCTGGTACGAGTCCACTACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 99.6259 |
| LEAP-Seq n confirming: | 799 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACGCAAGGAGCTGAGAG |
| Suggested primer 2: | TTAATAAATTGGCGGTTGGC |