Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.248739 |
Chromosome: | chromosome_2 |
Location: | 5088061 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g104900 | FAP204 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCCTGACCATGCTGCTGCTGCTGATGCCA |
Internal bar code: | CGGTCTGTTTCGGAAACATCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 645 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 84 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTACCGAGACATCGCTGA |
Suggested primer 2: | AATTGGTTGGCTTTGTTTGC |