| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248739 |
| Chromosome: | chromosome_6 |
| Location: | 7512012 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g300500 | CYA18,ADCY1 | Adenylyl cyclase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTATGTAACGTTGCGTGGCTTGCTGACCC |
| Internal bar code: | AGGTTAAGTATCGGCCTACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 98.7277 |
| LEAP-Seq n confirming: | 388 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCGGGTAAGGAGACCAT |
| Suggested primer 2: | CAAGGGGAGTTGGTTCGTTA |