| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248763 |
| Chromosome: | chromosome 3 |
| Location: | 2829645 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162366 | (1 of 2) 1.10.3.3 - L-ascorbate oxidase / Ascorbase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCACGGTCCGGCGTGCCGAATGGGCCA |
| Internal bar code: | ACGTTAACAGACTGCCACCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 99.511 |
| LEAP-Seq n confirming: | 2035 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGGTCTCCTGCTTCAG |
| Suggested primer 2: | CACTACCGGCAGTAACAGCA |