| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248764 |
| Chromosome: | chromosome 10 |
| Location: | 97592 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418300 | (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTAGCCTTTAACCTGCGTGCTGCCTCC |
| Internal bar code: | ATTGAGCGTGCCGTTCGACCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 99.6523 |
| LEAP-Seq n confirming: | 4012 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACATGGAAAGTTGTGTG |
| Suggested primer 2: | GCTGTTGCGGATGAACAGTA |