| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.248790 |
| Chromosome: | chromosome 16 |
| Location: | 6972350 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677205 | (1 of 4) PF00207 - Alpha-2-macroglobulin family (A2M) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATTCCATGGACTCCGCCGCTGGCACACC |
| Internal bar code: | GCGCCCCAAGCGGTGCAGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 140 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTGGCTTTTTGGTGATT |
| Suggested primer 2: | AGCAGCTGGGGCTATGTCTA |