Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.248891 |
Chromosome: | chromosome 16 |
Location: | 6105205 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g675850 | (1 of 1) K11729 - acyl-CoA dehydrogenase family member 10 (ACAD10) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACACACAGGTGCATCGCATACA |
Internal bar code: | AATATCTAGTCTCCACCGAGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 215 |
LEAP-Seq percent confirming: | 57.0439 |
LEAP-Seq n confirming: | 247 |
LEAP-Seq n nonconfirming: | 186 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCGTATGGTTGCGTATTG |
Suggested primer 2: | TGCCTTGCTCTTTACGTGTG |