| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.248937 |
| Chromosome: | chromosome 17 |
| Location: | 2944365 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719900 | PWD1 | Phosphoglucan water dikinase 1; (1 of 1) K15535 - phosphoglucan, water dikinase (PWD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATACTGCCCGCGCACCGGTGCCACGTCA |
| Internal bar code: | GGGTCCCGGCGGGGAGGAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 91.8239 |
| LEAP-Seq n confirming: | 146 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACAATTTCCGAGGTTCG |
| Suggested primer 2: | CACCCCTGACCCTCTTTACA |