Insertion junction: LMJ.RY0402.248966_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.248966
Chromosome:chromosome 17
Location:5749698
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g739150 CTPA5,TSP5 (1 of 8) 3.4.21.102 - C-terminal processing peptidase / Tsp protease; putative C-terminal peptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACGAACACACACATGCATACACACAG
Internal bar code:TCCATTAGTCTCTATCGTAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:200
LEAP-Seq percent confirming:99.2629
LEAP-Seq n confirming:4983
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTTATCGCTTGCATCTGTCG
Suggested primer 2:ATCAATCCGTTCTTTCCGTG