Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.249008 |
Chromosome: | chromosome 11 |
Location: | 373468 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467576 | (1 of 1) PTHR33403//PTHR33403:SF5 - FAMILY NOT NAMED // PROTEIN SPIRAL1-LIKE 2-RELATED | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCGTATTGCTGCTGCGCTGGTGCGGTG |
Internal bar code: | TGGGGCGGACCACTTCGATGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 553 |
LEAP-Seq percent confirming: | 97.4249 |
LEAP-Seq n confirming: | 454 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTCGGCAGTTACAATTGGG |
Suggested primer 2: | ATTACGTGGTGCTTTCCCAC |