Insertion junction: LMJ.RY0402.249027_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.249027
Chromosome:chromosome 16
Location:4940721
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g685450 DHC8 Axonemal Dynein Heavy Chain 8; (1 of 3) PTHR10676//PTHR10676:SF242 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // DYNEIN HEAVY CHAIN 3, AXONEMAL CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAACAACATGATGGAGGGCATCATCGCG
Internal bar code:ATCACTCGTTGGGACCCACCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:601
LEAP-Seq percent confirming:99.684
LEAP-Seq n confirming:1262
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCCCAAACTAAACCAAA
Suggested primer 2:CCAACAGCTGTATCTCGCAA