| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249031 |
| Chromosome: | chromosome_3 |
| Location: | 8094590 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g200950 | FAP273 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGGTTCTAGCTTTCGCAGTTTTCGCAATT |
| Internal bar code: | CCATGACGTGAAAGGTGGATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 97.7778 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGTGTGAATGCTCATGG |
| Suggested primer 2: | AACATTGAACTTTCGCCACC |