| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.249034 |
| Chromosome: | chromosome 8 |
| Location: | 342371 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358570 | MCP23 | (1 of 22) IPR018108//IPR023395 - Mitochondrial substrate/solute carrier // Mitochondrial carrier domain; Mitochondrial substrate carrier protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAAGTTCTGCAGTCTCAAGCGCGCAAGTA |
| Internal bar code: | GGTTGGACGCAGTTACGTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 98.4772 |
| LEAP-Seq n confirming: | 194 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAACTGTCTCGTTTCCGC |
| Suggested primer 2: | CGCTGCAACTGAATACCTGA |