Insertion junction: LMJ.RY0402.249034_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.249034
Chromosome:chromosome 8
Location:342381
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g358570 MCP23 (1 of 22) IPR018108//IPR023395 - Mitochondrial substrate/solute carrier // Mitochondrial carrier domain; Mitochondrial substrate carrier protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGTTGCCTTGCTGCTTTTGATACTATG
Internal bar code:AGTAAAACGGGGCGCTGCGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:270
LEAP-Seq percent confirming:99.2
LEAP-Seq n confirming:124
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCTAACTGTCTCGTTTCCGC
Suggested primer 2:CGCTGCAACTGAATACCTGA