Insertion junction: LMJ.RY0402.249050_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.249050
Chromosome:scaffold 24
Location:61373
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre24.g755597 Chlorophyll a /Pheophorbide a oxygenase-related protein; (1 of 2) 1.14.13.122 - Chlorophyllide-a oxygenase / Cholorophyll-b synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTTTTACAAGTCCACGGCACCATCGCAT
Internal bar code:AGCGAATCGCAGAGCGGGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:592
LEAP-Seq percent confirming:99.5122
LEAP-Seq n confirming:204
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCCTTCAGTCAACCCCT
Suggested primer 2:GAGTGACAGGCCATTGGTTT