| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.249090 |
| Chromosome: | chromosome 10 |
| Location: | 5976183 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462450 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCAAATCTCATGGCAGTTCTCAATTTCT |
| Internal bar code: | TCTATGTGTCCGGTGCGACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 99.4413 |
| LEAP-Seq n confirming: | 178 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTATGCGTTTTGGAAACT |
| Suggested primer 2: | TTGAGAGTGATGATGAGGCG |