| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249202 |
| Chromosome: | chromosome 3 |
| Location: | 3161943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165200 | APG7 | Autophagy-related protein, ubiquitin-activating enzyme E1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATATTCTATCTACCCCAATACTGAGGCC |
| Internal bar code: | GCATTCACGGACAGTAATGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 42.1569 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGACTACCTTGCTCGCTT |
| Suggested primer 2: | GAATTAGGCGTGCTGGGTAA |