| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.249221 |
| Chromosome: | chromosome 1 |
| Location: | 6669125 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047900 | (1 of 2) K11583 - serine/threonine-protein phosphatase 2A regulatory subunit B'' (PPP2R3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTCCCGCGCTGCCCCCTGCCCCTCGCC |
| Internal bar code: | GTAATTTCCAGTCGTATAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 99.3506 |
| LEAP-Seq n confirming: | 765 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTTAGTCCGAAGCGCTCA |
| Suggested primer 2: | TTCGACCTCAAGAAGCAGGT |