| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249225 |
| Chromosome: | chromosome 16 |
| Location: | 1944403 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656300 | DPR1 | (1 of 2) 1.17.1.8 - 4-hydroxy-tetrahydrodipicolinate reductase / Dihydrodipicolinate reductase; dihydropicolinate (DAP) reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTTCTAGTGTAAGTCGGCCACACATCC |
| Internal bar code: | GAGTCCTCTCCCGCGTGGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 99.4932 |
| LEAP-Seq n confirming: | 589 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCCAAGTCGTGGATGT |
| Suggested primer 2: | GTAAAGCGTAAGCAGCCAGG |