Insertion junction: LMJ.RY0402.249234_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.249234
Chromosome:chromosome 7
Location:4916803
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g346350 (1 of 2) PTHR10174//PTHR10174:SF113 - RETINALDEHYDE BINDING PROTEIN-RELATED // RETINALDEHYDE-BINDING PROTEIN 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCAAGCGCTTGTCACGGCCTAGTTGACT
Internal bar code:TTGAGTCCTAAGGTATAAATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:832
LEAP-Seq percent confirming:99.0291
LEAP-Seq n confirming:1428
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAACACTTCAACATGGGTCG
Suggested primer 2:TTGTGTGCTCCTTTTGTTGC