| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.249238 |
| Chromosome: | chromosome 12 |
| Location: | 6093409 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535750 | CPA1 | (1 of 2) 3.5.1.53 - N-carbamoylputrescine amidase; N-carbamoylputrescine amidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGTGGAGTCGCCGCCCATTAATCATCA |
| Internal bar code: | CAGGTGTGTGGCGCGCGGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 24.8654 |
| LEAP-Seq n confirming: | 693 |
| LEAP-Seq n nonconfirming: | 2094 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGATTCCTACCACCACCTC |
| Suggested primer 2: | TGCCTCCTCCTCCAACTCTA |