Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.249251 |
Chromosome: | chromosome_10 |
Location: | 3000552 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g440950 | FAP192 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACGTTCCGTGGGCGTAGGGGAGGTGCCAAA |
Internal bar code: | ACTGGTGTGTAGATAATAAGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 173 |
LEAP-Seq percent confirming: | 99.8704 |
LEAP-Seq n confirming: | 1541 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGTGGAGAGGAAGTGGTGC |
Suggested primer 2: | TTCTTTGTGTCTCGTCACGC |