Insertion junction: LMJ.RY0402.249255_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.249255
Chromosome:chromosome 6
Location:8419655
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g307100 AKC2 (1 of 2) PTHR10566//PTHR10566:SF72 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // PROTEIN KINASE FAMILY PROTEIN; conserved protein related to ABC1/COQ8 putative ser/thr kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCGTGAGGTACGGCAAGTGGGGTGCTGC
Internal bar code:GTCAGGTTACAGGTGCCCTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:337
LEAP-Seq percent confirming:99.2727
LEAP-Seq n confirming:273
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CATCTTCCTCACCCTACCCA
Suggested primer 2:CTGTTTGTTCAGCTCGTCCA