| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.249307 |
| Chromosome: | chromosome 7 |
| Location: | 4918594 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346400 | FKM6 | DNA methylase in the FkbM family; (1 of 3) K05302 - SET domain-containing protein 6 (SETD6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATCCACACGGTGCCTGCGTGTGGTGC |
| Internal bar code: | GACCTTAAATCAATCTGTCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 99.053 |
| LEAP-Seq n confirming: | 1569 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGTGTGTTCCTTCCTGT |
| Suggested primer 2: | GTTAGGAAAGCAGCGTCAGG |