| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249342 |
| Chromosome: | chromosome 4 |
| Location: | 3092286 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225500 | CSA6 | (1 of 1) PF15066 - Cancer-associated gene protein 1 family (CAGE1); conserved protein of unknown function | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCATGCATTCACACTCCAGGAGCGTGCA |
| Internal bar code: | TCTGAATGGTACGCCAACGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.4273 |
| LEAP-Seq n confirming: | 868 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGCGCATGTCCTTCACAG |
| Suggested primer 2: | CAGCCATGGTCACGACATAC |