| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.249372 |
| Chromosome: | chromosome 17 |
| Location: | 5875496 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739900 | (1 of 1) IPR012337//IPR027417 - Ribonuclease H-like domain // P-loop containing nucleoside triphosphate hydrolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTGAAGCAGGCAGTGGTAGACCTGCGA |
| Internal bar code: | GTGTCCACCCCGGCAATGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 86.372 |
| LEAP-Seq n confirming: | 938 |
| LEAP-Seq n nonconfirming: | 148 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCATTGAGGAGGTAGAGG |
| Suggested primer 2: | AAATGCTTCGTTGTGGAACC |