| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.249380 |
| Chromosome: | chromosome 1 |
| Location: | 4802353 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033200 | (1 of 3) IPR001510 - Zinc finger, PARP-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTCCAATCTCTGCGGGCTGTACTGCAT |
| Internal bar code: | GTGAATTTTATTGGGGGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 97.7621 |
| LEAP-Seq n confirming: | 830 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGTGACTCGCGGTGATA |
| Suggested primer 2: | AGCAGGAGGACGATGATGAC |