| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.249419 |
| Chromosome: | chromosome 14 |
| Location: | 1485049 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTTTAGCCTTGTCACTTGTCAGCATGT |
| Internal bar code: | TGGATGATTGGGAACATTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 99.6281 |
| LEAP-Seq n confirming: | 4286 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCCTCCAACTCATCCT |
| Suggested primer 2: | CGAGACACTGAAAGGCATCA |