| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.249426 |
| Chromosome: | chromosome 11 |
| Location: | 310230 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467563 | (1 of 10) PTHR10174 - RETINALDEHYDE BINDING PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAACAGGGCACAGTGAGCGGCAGGGGTA |
| Internal bar code: | ACGGATTTGCCCACTCCGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 459 |
| LEAP-Seq percent confirming: | 99.0567 |
| LEAP-Seq n confirming: | 16696 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAACCACGAGTCACAGGGC |
| Suggested primer 2: | ATCCACCTTGTTGTTAGCCG |