| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.249437 |
| Chromosome: | chromosome 16 |
| Location: | 2396599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660225 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTCGACCTGAATGACCTGGTAGTCTTG |
| Internal bar code: | ACTTTCTCGCAATTAAGATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 99.5211 |
| LEAP-Seq n confirming: | 8312 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTGCAGAACTGACATA |
| Suggested primer 2: | GCAAGAAGGAGTACCTTGCG |