Insertion junction: LMJ.RY0402.249442_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.249442
Chromosome:chromosome_12
Location:3385391
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g497000 FAP14 Flagellar Associated Protein antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TCTCGCCCTTGATCCTGTGTTCGCAGCGCG
Internal bar code:GCACCGCCGCACGTCGTAGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:608
LEAP-Seq percent confirming:99.1597
LEAP-Seq n confirming:118
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTATGCTACCGCCAGACCTC
Suggested primer 2:GCTTTTCATCGCGCTTTATC