| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.249444 |
| Chromosome: | chromosome 7 |
| Location: | 543836 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316200 | (1 of 726) IPR011009 - Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTGGCCGGGCACGCAGTGACAAGTGTT |
| Internal bar code: | CTGCATTGTTATGGGTTATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 81.3001 |
| LEAP-Seq n confirming: | 19160 |
| LEAP-Seq n nonconfirming: | 4407 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGAGCGCGTGTATGTC |
| Suggested primer 2: | TTCCAGTGGACCAGTAAGCC |