| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.249445 |
| Chromosome: | chromosome_4 |
| Location: | 2934478 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g224650 | FAP296 | Flagellar Associated importin alpha-like protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTGGGCACAGCCATCCTGACTTAGTATAA |
| Internal bar code: | ACCGTCCGTCGAATAGGTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 99.3127 |
| LEAP-Seq n confirming: | 289 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAGGTTAGAGCCAGGCA |
| Suggested primer 2: | TGAGCTGACTGGTATCGTGC |