Insertion junction: LMJ.RY0402.249452_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.249452
Chromosome:chromosome 3
Location:1123050
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g149001 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAAGACGGTTAGTACACCCAGAGGGAGA
Internal bar code:GTCGAACAGACTTAGTGCGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:319
LEAP-Seq percent confirming:99.2481
LEAP-Seq n confirming:132
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGGGAGGCAGAATGTGT
Suggested primer 2:AGCTAGTTTGGTCCCCGAAT